Differential live M. tuberculosis -, BCG-, and recombinant ESAT6- and CFP10- induced immunity in tuberculosis. - Related Articles
Differential live M. tuberculosis -, BCG-, and recombinant ESAT6- and CFP10- induced immunity in tuberculosis.
Clin Vaccine Immunol. 2009 May 13;
Authors: Hasan Z, Jamil B, Ashraf M, Islam M, Dojki M, Irfan M, Hussain R
The high prevalence of Mycobacterium tuberculosis makes it imperative to evaluate immune responses which could be predictive of infection. We investigated live Mycobacterium and recombinant antigen induced cytokine and chemokine responses in active tuberculosis (TB) as compared with healthy endemic controls (ECs). M. tuberculosis-, M. bovis BCG, ESAT6- and CFP10-induced responses were determined in peripheral blood mononuclear cells from pulmonary TB patients (n=38) and ECs (n=39). Cytokines IFNgamma and IL10, and chemokines CCL2, CCL3, CXCL9 were measured. M. tuberculosis- and BCG-induced IFNgamma secretion was significantly reduced (p=0.002, p<0.01), while IL10 induced by both virulent (p<0.01) and avirulent (p=0.002) mycobacteria was increased in TB patients. ESAT6-induced IFNgamma responses were increased in TB patients (p=0.013) when compared with the EC group. When Tuberculin Skin Test negative (TST, <10 mm) and TST positive donors were studied separately, both TST- and TST+ individuals showed increased IFNgamma responses to M. tuberculosis as compared with TB patients (p=0.037, p=0.006, respectively). However, only TST+ ECs showed reduced IFNgamma to ESAT6 (p=0.008) when compared with TB patients. M. tuberculosis -induced CCL2 (p=0.006), and CXCL9 (p=0.017) was greater in TB. CCL3 secretion in response to Mycobacterium- and antigen-stimulation was comparable between groups. While, ESAT6 induced chemokines did not different between TB patients and ECs, CFP10-induced CCL2 (p=0.01), and CXCL9 (p=0.001) was raised in TB. These data indicate differential host IFNgamma, CXCL9 and CCL2 responses to live mycobacteria and mycobacterial antigens and have implications for the identification of potential biomarkers of infection which could be used in TB diagnosis.
PMID: 19439524 [PubMed - as supplied by publisher] [Tuberculous Meningitis]